FastQCFastQC Report
Thu 8 Feb 2018
OD7_k4me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD7_k4me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35310847
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG14200.022.97019241
CTCGTAT17550.018.58598937
CGTATGC18450.017.92280239
TCTCGTA19100.017.31325536
ATGCCGT21100.015.67187942
TCGTATG22700.014.26992138
ACACGTC27000.012.9970618
TGCCGTC25300.012.89239743
ATCTCGT27000.012.24752535
TAGCCGG91200.012.2142691
CGTCTGA30350.011.85893311
GCCGTCT28150.011.66703944
TAGGCCG19100.011.3337381
TCGTATA28600.011.16880412
TGGCGAA28950.010.6710551
CTTCGTA36350.010.64408610
TGGCCGG57600.010.5700391
CGTATAA33100.09.78630613
GGCGAAA28800.09.7634172
TGGGCCG35800.09.7630171
Produced by FastQC (version 0.11.5)