FastQCFastQC Report
Thu 8 Feb 2018
OD8_k4me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD8_k4me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57409692
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG111100.011.6612581
TGGGGCG83200.010.0200531
AGGGGCG108550.09.9010251
TGGGCCG78300.09.8126031
TGGGGTT323350.09.4906481
AGGGCGG100850.09.2494541
AGGGTTT322500.09.0475641
AGGGGGG110600.08.9433661
AGGGCCG87200.08.8369261
AGGCGCC125300.08.5954471
GGGGTTT358350.08.5933052
AGGGCGC72750.08.5790341
AGGCCGG148150.08.5624551
TGGGGGG194850.08.5570271
TAGGCCG33250.08.4705191
AGGCGTG207400.08.4086031
TAGCCGA20050.08.3158941
GCGTGAG130750.08.3080883
TGGGCGG105500.08.2864951
TGGCCGG109150.08.2571061
Produced by FastQC (version 0.11.5)