FastQCFastQC Report
Thu 8 Feb 2018
OD9_k4me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD9_k4me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63636764
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG133550.011.747231
TGGGGCG112950.010.3973251
AGGGGCG140500.09.5938961
AGGGCGG132700.09.3764471
TGGGGTT351950.09.1008771
AGGGCCG103350.09.0076121
TAGGCCG37650.08.8606761
TGGCGAA56000.08.7748271
TGGCGGC129150.08.761521
AGGCGCC159500.08.7478261
AGGGTTT359850.08.6881061
TGGGCCG96200.08.599261
AGGGGGG133850.08.5549111
TGGGCGG126350.08.5453611
TGGCCGG128850.08.5020171
AGGGCGC95150.08.4572571
TAGCCAG384050.08.3695451
TGGGTCG40200.08.354691
AGGCGTG236250.08.3293781
AGGGGCC242100.08.2770791
Produced by FastQC (version 0.11.5)