FastQCFastQC Report
Thu 8 Feb 2018
YD10_k4me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD10_k4me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66127018
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG133750.010.3367341
TGGGGTT368150.08.3806541
AGGGGCG63850.08.3361931
TGGCGAA45800.07.8790011
AGGGTTT373950.07.83451751
AGGGCGG66000.07.72291371
TCCCGTT123350.07.67939141
TGGGCCG53300.07.6589291
AGGGCCG55150.07.52469641
TAGCCAG420750.07.4830381
GCGTGAG129800.07.4694763
CCAACGA125700.07.3730839
TAGGCGG20000.07.3299331
TCCAACG128950.07.3268138
TGGCCGG90250.07.29713341
CGGCTAA87450.07.254410320
TAGGCGT28200.07.1979711
CGTGAGC128600.07.15434464
TGGCCAG602800.07.1424851
GGGGTTT424550.07.1374442
Produced by FastQC (version 0.11.5)