FastQCFastQC Report
Thu 8 Feb 2018
YD11_k4me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD11_k4me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68502660
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG194100.012.6392021
TGGGCCG88750.09.8323861
TGGGGTT415700.09.7635741
AGGGTTT397300.09.6595591
TAGGCCG44450.09.4353391
TAGCCAG532800.09.2639861
TGGCGAA60350.09.0791531
TCCCGTT120100.08.9367641
AGGGGCG105400.08.9209641
GGGGTTT489750.08.8694332
TGGGGCG90450.08.7501371
AGGCGCC159700.08.6833331
TACTCGG235900.08.62059937
AGGGGCC262550.08.5453011
AGGGCGG106700.08.5164171
TGGGTCG33700.08.4974791
AGGGCCG87750.08.4026631
TGGCCGG136450.08.3782011
CTACTCG243800.08.359714536
AGGCCGG183650.08.3489961
Produced by FastQC (version 0.11.5)