FastQCFastQC Report
Thu 8 Feb 2018
YD12_k4me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD12_k4me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50542287
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG114000.012.6961621
TATGCCG14450.011.20987941
TCGTATA38850.010.88414812
TGGCCGG100350.010.6488631
AGGGGCG86700.010.6092181
TGGGGTT298950.010.2787321
TCCCGTT103600.010.2059961
TGGGGCG71900.010.1278181
TGGGCCG71150.09.9494041
TAGGCCG30700.09.9137441
AGGGCCG72800.09.8477731
CTTCGTA49700.09.8204410
AGGCGCC125100.09.7494971
TAGCCGA17400.09.7174941
AGGGCGG86750.09.5116071
ACTAGAC135250.09.48296922
CGTATAA42500.09.4730913
AGGGTTT295600.09.4266271
TAGGCGC17050.09.3880681
TGGCGTT32650.09.3216511
Produced by FastQC (version 0.11.5)