FastQCFastQC Report
Thu 8 Feb 2018
YD14_k4me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD14_k4me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55567342
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG161850.013.7205611
TGGGGTT340350.010.5057191
TAGGCCG31850.010.4053391
TCCCGTT103750.09.7567821
TAGCCAG455300.09.7300081
TAGCCGA21800.09.30752851
GGGGTTT394550.09.2350942
AGGGTTT339850.09.1546161
CCAACGA113700.09.0205539
TGGCGAA49200.08.9813031
TGGCCAG612400.08.960531
TACTCGG204300.08.83238237
TGGCCGG93350.08.8150941
TCCAACG119650.08.7599578
AGCCGGG243600.08.6330192
CTACTCG213950.08.61278236
CGGGCGT89650.08.5050685
CAACGAA122900.08.49170410
TAGGCGG21650.08.43481351
TGGGCCG56700.08.3897251