FastQCFastQC Report
Thu 8 Feb 2018
YD15_k4me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD15_k4me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73964761
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG188600.011.8307071
TAGGCCG43700.010.9853861
TCCCGTT154000.010.1275071
TGGGCCG82250.09.9195011
TGGGGTT435500.09.8329231
AGGGCCG87850.09.518081
TGGCCGG133850.09.4799761
GGGGTTT497050.09.4080212
TAGCCAG539100.09.3354791
TGGCCAG770700.09.1766621
AGGGTTT446600.09.0838721
AGGGGCG97350.09.0291281
CCAACGA168800.08.9537519
TGGGGCG81750.08.904961
TCCAACG174950.08.7675578
TCGTATA55500.08.67219412
TGGCGTT48600.08.62568951
CCCGTTT201650.08.593012
AGGGGGG129450.08.5660421
CAACGAA181450.08.52785110
Produced by FastQC (version 0.11.5)