FastQCFastQC Report
Thu 8 Feb 2018
YD16_k4me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD16_k4me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67358664
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG164650.011.7173721
TAGGCCG48100.011.7142131
TCGTATA50800.011.15687112
TGGGGCG132900.010.63312151
CTTCGTA62900.010.54816510
TGGGCCG130050.010.5022041
CGTATAA54500.010.27562913
AGGGCCG136650.010.1928831
AGGGGCG166950.010.13845351
ACTAGAC176700.09.98022922
ATCTTCG80400.09.9586298
TGGCCGG172400.09.8310241
TCCCGTT135000.09.7832251
AGGGCGG164700.09.6474751
TGGGGTT420550.09.6251351
AGGCGCC207150.09.6180161
AGGGTTT392350.09.6161221
AGGGGGC295400.09.3845361
AGGGGCC305350.09.3444551
TGGGCGG169950.09.3096681
Produced by FastQC (version 0.11.5)