FastQCFastQC Report
Thu 8 Feb 2018
YD17_k4me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD17_k4me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96630314
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG235450.012.6193771
TAGGCCG63150.011.0391591
AGGGGCG235300.010.5372341
TGGGGCG193750.010.3982581
TGGGCCG165450.010.199661
TGGGGTT601250.010.1874521
AGGGCCG188200.09.4701811
AGGGTTT555400.09.3508331
AGGGCGG234550.09.3493561
GGGGTTT668350.09.3002052
TGGGCGG227700.09.2243861
TCCCGTT161150.09.1698411
AGGGCGC157750.09.1672581
AGGCGCC271850.09.15370751
TGGCCGG231850.08.9035841
AGGCCGG308850.08.8460091
TAGCCAG641800.08.7915371
TGGCGGC211450.08.7489711
AGGCGCG159700.08.6315161
AGGGGGG223550.08.5983631
Produced by FastQC (version 0.11.5)