FastQCFastQC Report
Thu 8 Feb 2018
YD18_k4me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD18_k4me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77357833
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG217550.013.8181231
TGGGCCG92250.010.4629811
TCCCGTT147650.010.1570191
TGGGGTT472100.010.0218881
TAGGCCG44350.010.01731
AGGGGCG96800.09.8779891
TGGCCGG141500.09.8175321
AGGGTTT473800.09.7431821
TGGGGCG85750.09.4940411
TGGCGAA69300.09.4697341
TAGCCAG612350.09.3359021
GGGGTTT544700.09.2101112
TAGGGCG20100.09.200171
TAGCCGA28100.09.1490621
TGGCCAG828450.09.05386451
AGGCCGG191400.08.8721951
CGGGCGT130250.08.72235
AGGGCGG99600.08.626681
CCCGTTT191250.08.58694652
TGGGCGG115200.08.574321
Produced by FastQC (version 0.11.5)