FastQCFastQC Report
Thu 8 Feb 2018
YD19_k4me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD19_k4me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20988202
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG9250.023.3536441
CTCGTAT10850.019.28843337
TCGTATG11650.017.57687238
TCTCGTA12150.017.03927636
CGTATGC12500.016.38152739
ATGCCGT14000.015.91155442
ACACGTC16400.013.7180468
ATCTCGT16050.012.61820935
TAGCCGG50700.012.4417791
CGTCTGA21150.011.16915111
GTATGCC22650.010.13344340
TGCCGTC21600.010.00062343
AGGGGCG60100.09.971021
TGGGGCG52050.09.7385481
AGGGCCG47250.09.7265951
AGGCGCC66950.09.522881
TGGGGTT133400.09.47413351
ACGTCTG26300.09.23860110
TCGTATA12200.09.22043612
CACGTCT26150.09.1195289
Produced by FastQC (version 0.11.5)