FastQCFastQC Report
Thu 8 Feb 2018
YD1_k4me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD1_k4me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61957295
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG145800.010.7821011
TGGGGTT366900.09.2946331
AGGCGCC125600.09.0683831
TAGCCGA21400.08.8530651
TAGGCCG35850.08.6819661
TGGCCGG107500.08.6650241
TGGGCCG68900.08.5110331
TCCCGTT104600.08.4524481
AGGGGCG80350.08.4490671
TCCAACG111350.08.404118
GGGGTTT425500.08.3322872
TGGGGCG72050.08.2328441
TGGCGAA53400.08.1938611
ACGCCCG55250.08.18417415
AGGGTTT367650.08.1407521
AGGGCCG71250.08.1353521
TAGGCGA15300.08.1077331
ACCACGC150700.08.01585112
CCAACGA112650.07.98762049
TAGCCAG438450.07.9527541
Produced by FastQC (version 0.11.5)