FastQCFastQC Report
Thu 8 Feb 2018
YD20_k4me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD20_k4me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20702178
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG66100.013.7353211
TGGGGTT135300.09.9405811
TAGCCAG167100.09.5588361
AGGGTTT128150.09.5458911
TGGGGCG30200.09.3247741
AGGGGCG35600.09.3025621
TGGCCAG246300.09.1559811
TGGCGAA17400.09.06325151
TGGCGAC11450.09.0508131
GGGGTTT158700.08.8599792
AGGGCGG36800.08.815561
ACGGGGT56500.08.7722191
AGGGCCG28650.08.7283791
GTAGTCC136350.08.69591926
AGGGCGC24400.08.6790711
CTACTCG86000.08.63494236
TCGAACT69350.08.62927215
TGGGCCG30850.08.6171181
TAGCCGA8900.08.6064511
TAGGCCG14200.08.5672341
Produced by FastQC (version 0.11.5)