FastQCFastQC Report
Thu 8 Feb 2018
YD21_k4me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD21_k4me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19128293
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG53450.011.8440961
TAGGCGA5500.010.6501071
AGGGGCG44050.09.8708531
TGGGGTT120150.09.6379061
TGGGGCG39250.09.4134221
AGGCGAC12600.09.2977131
TAGGCCG13650.09.2426971
AGGGCGG43950.09.2269251
TAGCCAG142900.09.112531
AGGGTTT111500.09.0318351
TAGCGAA4551.2534656E-88.91260051
GGGGTTT139200.08.8567092
TGGCCGG49050.08.7268581
AGGCCGG65050.08.6583681
TGGCGAA19050.08.6331891
AGGCTCG21500.08.59248351
TGGCCGT17500.08.4966791
TAGCCGA8050.08.3959281
TGGGCCG35200.08.3843991
AGGGGGG44650.08.3758711
Produced by FastQC (version 0.11.5)