FastQCFastQC Report
Thu 8 Feb 2018
YD2_k4me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD2_k4me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41203900
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG91600.011.393341
TAGGCCG18950.08.68318751
TAGCCCG10050.08.5228161
TGGGGTT227850.08.3692561
GATCGGA11450.08.2681691
TCCCGTT69500.08.0756981
TAGGCGT17100.08.04081
TATGCCG8400.08.03240741
TGGGCCG29750.08.0312741
TAGGCGC8800.07.94043971
TAGGCGG11800.07.8319051
AGGGGCG30350.07.79823161
ACCGCGC22850.07.77579212
TAGCCAG281550.07.76572041
AGCGTCG4101.808006E-57.69679161
CGGGCGT49800.07.67775735
CGCCCGG45200.07.613117716
CTACTCG122250.07.516701736
TACTCGG120550.07.49192137
TGGCGAA29550.07.47539471
Produced by FastQC (version 0.11.5)