FastQCFastQC Report
Thu 8 Feb 2018
YD3_k4me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD3_k4me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67463343
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG80550.010.29422951
GATCGGA17200.09.956271
ACCGCGC35450.08.81728812
ACGCCCG27400.08.6996115
TCCCGTT156800.08.6509141
CGCCCGG54050.08.52913416
CGTGAGC110150.08.453074
CCAACGA169200.08.4393669
TGGGGCG28900.08.3425251
TGGGGTT320900.08.3417721
TCCAACG172250.08.3290978
CACCGCG38450.08.24630211
GCGTGAG111900.08.2002923
TGGGACG30850.08.1803981
AGGGTTT366900.08.0021781
AGGCGTG168550.07.9676281
CTCGTAT24500.07.89446342
TGGCGAA37200.07.87430051
CGAAATC98100.07.862516413
TGGCCAG478800.07.79794031
Produced by FastQC (version 0.11.5)