FastQCFastQC Report
Thu 8 Feb 2018
YD4_k4me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD4_k4me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68399003
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG177200.012.8558271
TGGGGTT402250.09.7412821
TGGCGAA62450.09.3450141
AGGGGCG80350.09.0859791
TAGGCCG34400.08.9737661
TACTCGG204400.08.799746537
GGGGTTT452650.08.7262372
TAGCCAG520750.08.6971991
TGGCCGG108600.08.5690541
TAGCCGC15050.08.5339671
CTACTCG216900.08.47966736
AGGGTTT407200.08.3999481
GGCGAAA58650.08.3609092
GTAGTCC376800.08.30763226
TGGGGCG68200.08.2267361
AGGCGCC121550.08.1751591
AGGCGAC29250.08.1656851
TAGCCCG21800.08.1655081
TGGCCAG686900.08.14180851
TAGCCGA24550.08.0768831
Produced by FastQC (version 0.11.5)