FastQCFastQC Report
Thu 8 Feb 2018
YD5_k4me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD5_k4me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69223942
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG167450.012.1986321
TAGGCCG36600.010.6452051
TGGGGTT425850.09.8683371
TCCCGTT112400.09.4372091
TGGCCGG122400.09.1445841
GGGGTTT478050.09.0151162
AGGGTTT404050.08.7063261
TGGGGCG78150.08.6165061
TGGGCCG74050.08.6069711
AGGCGCC140150.08.5970541
AGGGCGG90250.08.5592541
TAGGCGC21500.08.3799321
TGGCGAA58400.08.3682741
AGGGGCG88550.08.3675121
TGGCCAG706350.08.3599131
CCCGTTT143450.08.29044152
TAGCCAG496900.08.2760051
CCAACGA114100.08.2749049
TCCAACG117100.08.2546568
TGGGTCG33650.08.1651421
Produced by FastQC (version 0.11.5)