FastQCFastQC Report
Thu 8 Feb 2018
YD6_k4me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD6_k4me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80239214
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG172250.010.6007571
AGGGGCG182750.010.1767161
AGGGCCG146650.09.6535851
TGGGGCG147050.09.3513841
AGGGCGG180100.08.9746291
TGGGGTT452600.08.920561
TCCCGTT143100.08.8060921
TGGCGGC174200.08.7609581
TGGGCCG124350.08.7017491
TAGGCCG48200.08.3717661
TGGGCGG168600.08.3433051
AGGCGCC208750.08.2936521
TGGCCGG168300.08.29120351
GGGGTTT501150.08.2085052
TAGGCGG40450.08.1923761
TGGCGAA74850.08.1919671
AGGGTTT441500.08.1491681
AGGCGTG295550.08.1079931
AGGCGCG128450.08.0905481
CCAACGA139450.08.0804839
Produced by FastQC (version 0.11.5)