FastQCFastQC Report
Thu 8 Feb 2018
YD7_k4me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD7_k4me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53557541
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG14900.013.43784341
TAGCCGG109500.011.2046061
TGGGGCG89950.010.3051061
CTCGTAT20000.010.23616437
TAGGCCG31350.010.21556951
AGGGGCG105700.09.96445751
CGTATGC21350.09.79965839
TAGCCCG21850.09.3929441
AGGGCCG83850.09.3871581
AGGCGCC130550.09.2770351
TCTCGTA22650.09.1378836
TGGGGTT313900.09.1176331
TGGCCGG107450.09.0675331
TGGGCCG80200.08.9988581
TAGGCGC19400.08.9516021
AGGGCGG101850.08.791061
TGGCGGC99850.08.7638611
TCGTATG24200.08.73852938
AGGCGCG69650.08.6781161
TGGGCGG103700.08.5907311
Produced by FastQC (version 0.11.5)