FastQCFastQC Report
Thu 8 Feb 2018
YD8_k4me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD8_k4me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44431554
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG90350.012.1144991
TAGGCGC15550.010.71762851
AGGGGCG87400.010.6165441
TGGGGCG75700.010.3235941
TGGGGTT258650.09.9089381
AGGGCCG68800.09.591281
TAGGCCG26300.09.5909061
TGGGCCG61900.09.4597591
AGGGCGG88550.09.3087181
TAGCCGT11650.09.0859291
AGGCGCC105150.09.0385881
TAGCCAG282650.08.98788451
TGGCGAA37650.08.972711
AGGGGGC159600.08.8900671
GGGGTTT289550.08.8511222
TCCCGTT76350.08.7018241
TGGCCGG84450.08.6939131
TAGGGCG15300.08.6847631
TGGGGCT231200.08.5916811
AGGCCGG117550.08.4683151
Produced by FastQC (version 0.11.5)