FastQCFastQC Report
Thu 8 Feb 2018
YD9_k4me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD9_k4me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43024545
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG74450.011.4949381
TGGGCCG60900.09.688841
AGGGCGG85900.09.6218961
TAGGCGG19850.09.5302991
TAGGCCG24300.09.3605821
TAGCCGC15750.09.2943841
CGGCTAA62700.09.17855120
AGGCGAC28850.09.1333021
AGGGGCG88500.09.08474351
TGGCGGC80500.08.8964971
TGGGGTT244800.08.8961651
AGGGCCG69000.08.8778461
TAGCGCG7400.08.82581
AGGCGTG152850.08.82571
GCGTGAG97150.08.7468913
AGGGGGC152200.08.7006251
AGGCGGC91900.08.6506151
CGTGAGC95900.08.6499274
AGGCGCC99700.08.6063221
GGCGTGA141950.08.599432
Produced by FastQC (version 0.11.5)