FastQCFastQC Report
Mon 7 May 2018
OD10_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD10_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103585741
Sequences flagged as poor quality0
Sequence length150
%GC51

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG4974930.4802717007160281Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT241250.033.6002581
CGCGATC608350.025.3602431
CGACATC396350.024.7088152
GCGCGAT460950.022.1170981
CGATCCC739200.021.0907023
CCCACTA927300.017.856627
CGGCGAT289850.017.1945341
CACTACT946100.017.1431399
CCCTACG416400.016.4366939
TACCCTA430100.016.2655587
CCACTAC1053450.016.195788
ATCCCAC1180800.014.8158065
TACTCGG936850.014.727557
ACTACCC543800.014.5978675
GCACTCG700850.014.4467883
TCGCCGA610000.014.3681037
CGCCGAA605500.013.8215538
CGGCGCT682850.013.6848381
CGGCAAT230950.013.6142641
CTTGACT849850.013.608791
Produced by FastQC (version 0.11.3)