FastQCFastQC Report
Mon 7 May 2018
OD3_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD3_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77849882
Sequences flagged as poor quality0
Sequence length150
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG5806800.7458970843398324Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT181650.025.630311
CGCGATC353550.025.030641
GCGCGAT261000.022.6979371
CGACATC301800.018.9391572
CGATCCC461850.018.8907073
CCCACTA597050.015.9631387
CGGCGAT217850.015.9527781
CACTACT600450.015.0691819
CCGAAAC333300.014.5845741
CGAATCC545800.013.8089193
CCACTAC705800.013.7585058
CGGCAAT176750.013.6474661
TACCCTA307450.013.626667
CCCTACG290600.013.4751789
TCGCCGA496800.013.4319517
GCCGAAT535700.013.4128151
GCACTCG575250.013.0519143
CGAAACC385900.012.9835612
AATCCCG553900.012.9572015
CGGCGCT492000.012.8009911
Produced by FastQC (version 0.11.3)