FastQCFastQC Report
Mon 7 May 2018
OD5_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD5_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106710982
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGATAATCTCGTATG3749290.3513499669602891TruSeq Adapter, Index 20 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT240900.025.2059021
CGCGATC513000.023.7153361
GCGCGAT382500.022.1905441
CGACATC405200.019.4339732
CGATCCC678400.018.5468483
CGGCGAT290050.018.4085041
CCCACTA848400.015.4748747
CGGCAAT240450.015.1759811
TTCGCTC320550.015.1572952
CACTACT873300.014.6050559
CGGCGCT712600.013.8352571
CCGGAAT265100.013.5185731
CCACTAC994000.013.418138
TACCCTA381500.013.0183957
CGGCACT497650.012.8138151
ATCCCAC1144900.012.7497925
TCGCTCG192650.012.479353
TCGATCT622000.012.440353
TACTCGG732400.012.422297
CCGCGCT906350.012.1983931
Produced by FastQC (version 0.11.3)