FastQCFastQC Report
Mon 7 May 2018
YD1_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD1_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97801958
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGGCGTATCTCGTATG5123730.5238882845269826TruSeq Adapter, Index 20 (97% over 38bp)
CGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCG1059770.10835877130394465No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT246400.031.693841
CGCGATC603050.026.4426461
GCGCGAT441400.023.512731
CGACATC397050.023.374832
CGATCCC740000.022.0962833
CCCACTA886900.019.0936347
CACTACT905600.018.1828049
TCGCTCG183150.017.8870333
CGGCGAT288000.017.3106731
CCACTAC1024200.016.9345478
TACCCTA377750.016.8799277
TTCGCTC306450.016.1177792
CCCTACG388550.015.9106379
ATCCCAC1192600.015.690245
CGGCAAT225050.014.9409711
CGGCGCT668050.014.7402451
ACTACCC525050.014.5708095
GCGATCC1111550.014.1275682
TTGACTA522750.013.8699882
TACTCGG681650.013.6936697
Produced by FastQC (version 0.11.3)