FastQCFastQC Report
Mon 7 May 2018
YD4_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD4_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86272150
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATATCTCGTATG6797360.7878973689655353TruSeq Adapter, Index 18 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT214450.027.5427511
CGCGATC503400.025.1635061
GCGCGAT369100.021.7389471
CGACATC358750.020.9489272
CGATCCC627250.020.9339663
CGGCGAT264000.018.5015051
CACTACT753450.017.511149
CCCACTA772400.017.5101937
TTCGCTC271500.016.2003572
CCACTAC866150.015.930728
TACCCTA315250.015.891347
TCGCTCG173350.015.3654473
CGGCAAT201300.014.7979391
TACTCGG625450.014.7422227
CGGCGCT605700.014.6334241
ATCCCAC1022300.014.590275
CCCTACG340250.014.3006239
ACTACCC431050.013.877955
CTACTCG732650.012.9977956
CTTGACT712600.012.9504881
Produced by FastQC (version 0.11.3)