FastQCFastQC Report
Mon 7 May 2018
YD4_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD4_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86272150
Sequences flagged as poor quality0
Sequence length150
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG6872030.796552537522248Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATC490950.025.731681
GCGACAT223050.024.554921
GCGCGAT348700.020.8793741
CGATCCC637000.020.2963393
CGACATC373200.018.8265422
CCCACTA759550.017.410037
CACTACT761050.017.0253899
CGGCGAT271400.016.9926431
CCACTAC875650.015.6853728
TACCCTA322300.015.6568537
CGGCAAT203350.014.2420691
ATCCCAC1020900.014.201295
CGGCGCT604850.013.5644621
TACTCGG647600.013.5612517
TTCGCTC272750.013.4870982
CCCTACG359500.013.4557559
CTTGACT694150.013.213691
ACTACCC462850.013.1421975
TTGACTA434550.013.1038552
TCGCTCG175950.013.0512523
Produced by FastQC (version 0.11.3)