FastQCFastQC Report
Mon 7 May 2018
YD7_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD7_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85207802
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGTCTGATCTCGTATG10076761.1826100149843086TruSeq Adapter, Index 15 (97% over 38bp)
CGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCG1025670.12037277994801462No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATC570500.028.4295671
GCGACAT203750.027.3745571
CGATCCC706650.023.3242443
GCGCGAT422750.023.135741
CGACATC329350.021.5787182
CACTACT825300.019.9567249
CCCACTA855500.019.5720047
CCACTAC931700.018.2725728
CGGCGAT250950.017.3251741
ATCCCAC1078650.016.9043885
TTCGCTC265100.016.5686172
TACTCGG648000.015.7079677
TCGCTCG165150.015.6524393
GATCCCA1205100.014.579374
TACCCTA317650.014.5716147
CTCGCTA118200.014.3767492
CGGCAAT197450.014.1423151
TCGCTAT125400.013.8958133
CGTGCCT497400.013.8895421
CTACTCG764300.013.8357526
Produced by FastQC (version 0.11.3)