FastQCFastQC Report
Mon 7 May 2018
YD7_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD7_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85207802
Sequences flagged as poor quality0
Sequence length150
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG10023781.1763922745008726Illumina Single End PCR Primer 1 (100% over 50bp)
CGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCG922550.1082706017930142No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATC543050.027.33841
GCGACAT216300.026.6280561
GCGCGAT399100.024.4565931
CGATCCC702300.021.4770363
CGACATC347000.020.5516452
CACTACT816100.018.6559269
CCCACTA845050.018.6058737
CCACTAC934450.016.864318
CGGCGAT250750.016.1898291
ATCCCAC1080000.015.4585845
TACCCTA326900.014.8803387
TTCGCTC258550.014.6400852
TACTCGG672250.014.6325067
CGGCAAT202550.014.4710881
GATCCCA1161700.013.8882454
CGGCGCT567700.013.8501431
GAGCGTC11520050.013.610299
CCCTACG347200.013.5324989
CGTGCCT511500.013.5238331
TCGCTCG167050.013.4835293
Produced by FastQC (version 0.11.3)