FastQCFastQC Report
Mon 7 May 2018
YD9_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD9_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91724961
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTTAACTATCTCGTATG6384370.6960340926173847TruSeq Adapter, Index 12 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT211000.026.6608411
CGCGATC477400.022.3553071
GCGCGAT368600.020.4207691
CGACATC370800.019.7350852
CGATCCC612950.017.4559573
CGGCGAT290750.017.1346871
CGGCAAT219650.015.8339981
CCCACTA743950.015.3399547
CACTACT758450.014.5719619
CGGCGCT668900.014.0850991
CCACTAC879850.013.5018168
TACTCGG666500.013.4194777
TACCCTA329850.012.4125327
CGGCACT452400.012.3707231
ATCCCAC998000.012.2785975
CTACTCG761400.012.2290156
ACTACCC444400.011.8684655
CGGCAGT469700.011.6379891
CCGAAAC283250.011.5894451
CGATCCA262500.011.5400254
Produced by FastQC (version 0.11.3)